Detailed information of TA system TA115327

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 108083..108336
• Replicon: plasmid p2b2
• Accession: NZ_CP034125
• Organism: Klebsiella pneumoniae strain BJCFK909

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: EH202_RS29705
• Protein ID: WP_001312851.1
• Coordinates: 108187..108336 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	108083..108142 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH202_RS29655	103465..103809	-	345	Protein_136	IS6-like element IS26 family transposase	-
EH202_RS29665	103861..104565	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
EH202_RS29670	104629..104790	+	162	Protein_138	DNA helicase	-
EH202_RS29675	104810..105556	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
EH202_RS29680	105611..106171	+	561	WP_000139328.1	fertility inhibition protein FinO	-
EH202_RS29685	106303..106503	+	201	WP_015059022.1	hypothetical protein	-
EH202_RS29690	106889..107488	+	600	WP_032083981.1	hypothetical protein	-
EH202_RS29695	107652..107882	+	231	WP_001736714.1	hypothetical protein	-
-	108083..108142	-	60	NuclAT_1	-	Antitoxin
-	108083..108142	-	60	NuclAT_1	-	Antitoxin
-	108083..108142	-	60	NuclAT_1	-	Antitoxin
-	108083..108142	-	60	NuclAT_1	-	Antitoxin
EH202_RS29705	108187..108336	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
EH202_RS29710	108620..108868	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..109179	109179

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T115327 WP_001312851.1 NZ_CP034125:108187-108336 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T115327 NZ_CP034125:108187-108336 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT115327 NZ_CP034125:c108142-108083 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin