Detailed information of TA system TA115322

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30204..30474
• Replicon: plasmid p2b2
• Accession: NZ_CP034125
• Organism: Klebsiella pneumoniae strain BJCFK909

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EH202_RS31465
• Protein ID: WP_001312861.1
• Coordinates: 30316..30474 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30204..30267 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH202_RS29040	25915..26442	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
EH202_RS29045	26500..26733	+	234	WP_000006003.1	DUF905 family protein	-
EH202_RS29050	26794..28817	+	2024	Protein_36	ParB/RepB/Spo0J family partition protein	-
EH202_RS29055	28886..29320	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EH202_RS29060	29317..30036	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	30048..30272	+	225	NuclAT_0	-	-
-	30048..30272	+	225	NuclAT_0	-	-
-	30048..30272	+	225	NuclAT_0	-	-
-	30048..30272	+	225	NuclAT_0	-	-
-	30204..30267	-	64	-	-	Antitoxin
EH202_RS31465	30316..30474	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
EH202_RS31810	30712..31089	-	378	Protein_40	hypothetical protein	-
EH202_RS29085	31389..31685	+	297	WP_001272251.1	hypothetical protein	-
EH202_RS29090	31796..32617	+	822	WP_001234445.1	DUF945 domain-containing protein	-
EH202_RS29095	32914..33561	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
EH202_RS29100	33838..34221	+	384	WP_000124981.1	relaxosome protein TraM	-
EH202_RS29110	34502..35206	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..109179	109179

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T115322 WP_001312861.1 NZ_CP034125:30316-30474 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T115322 NZ_CP034125:30316-30474 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT115322 NZ_CP034125:c30267-30204 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin