Detailed information of TA system TA115289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 12021..12544
• Replicon: plasmid pSTA491
• Accession: NZ_CP034112
• Organism: Staphylococcus epidermidis strain CDC120

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: C5QR05
• Locus tag: EH162_RS12310
• Protein ID: WP_002441971.1
• Coordinates: 12021..12287 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A8I0W7P8
• Locus tag: EH162_RS12315
• Protein ID: WP_002502803.1
• Coordinates: 12287..12544 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH162_RS12280	8258..9028	-	771	WP_124726236.1	CPBP family intramembrane metalloprotease	-
EH162_RS12285	9053..9601	-	549	WP_124726237.1	recombinase family protein	-
EH162_RS12290	9837..10388	-	552	WP_050961398.1	hypothetical protein	-
EH162_RS12295	10420..10671	-	252	WP_064658603.1	hypothetical protein	-
EH162_RS12300	10729..11121	-	393	WP_124726238.1	hypothetical protein	-
EH162_RS12945	11219..11386	-	168	WP_154804173.1	hypothetical protein	-
EH162_RS12305	11834..12004	-	171	WP_186308772.1	hypothetical protein	-
EH162_RS12310	12021..12287	-	267	WP_002441971.1	Txe/YoeB family addiction module toxin	Toxin
EH162_RS12315	12287..12544	-	258	WP_002502803.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EH162_RS12320	12722..13021	-	300	WP_124726240.1	hypothetical protein	-
EH162_RS12325	13048..13272	-	225	WP_124726241.1	hypothetical protein	-
EH162_RS12330	13432..13830	+	399	WP_124726242.1	YolD-like family protein	-
EH162_RS12340	15035..15697	+	663	WP_124726243.1	hypothetical protein	-
EH162_RS12350	16119..16523	+	405	WP_002502797.1	hypothetical protein	-
EH162_RS12355	17098..17376	+	279	WP_121826863.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..46728	46728

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10692.34 Da
• Isoelectric Point: 10.2282

>T115289 WP_002441971.1 NZ_CP034112:c12287-12021 [Staphylococcus epidermidis]
MAKYKINIKNSAKTDLKKIKRSHLQEQFLKIVEILKYDPYQQSQSFEKLHPKHLGRYSRRINHQHRVVYTIDEENKEVLI
LAAWSHYE

Nucleotide

• Length: 267 bp

>T115289 NZ_CP034112:c12287-12021 [Staphylococcus epidermidis]
ATGGCTAAATATAAAATCAACATAAAGAATTCAGCGAAGACAGATTTAAAGAAGATAAAACGTTCTCACTTACAAGAACA
ATTTTTGAAAATCGTAGAAATACTAAAATATGATCCATATCAACAAAGTCAATCCTTTGAAAAATTGCACCCTAAACATT
TAGGACGATACTCAAGACGTATCAATCATCAACATAGAGTTGTCTATACAATTGATGAGGAAAATAAAGAAGTATTAATA
TTAGCAGCTTGGTCTCATTATGAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9955.14 Da
• Isoelectric Point: 5.1750

>AT115289 WP_002502803.1 NZ_CP034112:c12544-12287 [Staphylococcus epidermidis]
MKSKTPTEARKDFYQLLKRVNSDHKPVYIEGKNDESNAVIIGMEDWRSIKETIYLEATGTMQKVREREQDNSGYTSIDDI
DWARL

Nucleotide

• Length: 258 bp

>AT115289 NZ_CP034112:c12544-12287 [Staphylococcus epidermidis]
ATGAAAAGTAAAACACCAACAGAAGCTCGTAAAGATTTTTATCAATTATTAAAACGTGTGAATAGTGATCATAAACCAGT
ATATATTGAGGGCAAAAATGATGAGAGTAATGCTGTGATTATAGGTATGGAAGATTGGCGTAGTATTAAAGAAACTATAT
ATCTTGAAGCTACAGGAACAATGCAAAAAGTTAGAGAACGTGAACAAGACAACAGTGGTTATACAAGCATAGATGATATT
GATTGGGCTAGACTTTAA