Detailed information of TA system TA115274

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69919..70172
• Replicon: plasmid pRCAD0514EC-2
• Accession: NZ_CP034108
• Organism: Escherichia coli strain RCAD0514

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: EGA47_RS23740
• Protein ID: WP_001312851.1
• Coordinates: 69919..70068 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	70113..70172 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGA47_RS23705	65226..65330	-	105	WP_032409716.1	hypothetical protein	-
EGA47_RS23710	65560..65748	+	189	WP_000957857.1	hypothetical protein	-
EGA47_RS23715	65758..66957	+	1200	WP_000948429.1	IS91 family transposase	-
EGA47_RS23720	67121..67279	-	159	WP_047667301.1	membrane protein	-
EGA47_RS23725	68218..69075	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
EGA47_RS23730	69068..69142	-	75	WP_001375168.1	RepA leader peptide Tap	-
EGA47_RS23735	69387..69635	-	249	WP_000083837.1	replication regulatory protein RepA	-
EGA47_RS23740	69919..70068	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	70113..70172	+	60	NuclAT_1	-	Antitoxin
-	70113..70172	+	60	NuclAT_1	-	Antitoxin
-	70113..70172	+	60	NuclAT_1	-	Antitoxin
-	70113..70172	+	60	NuclAT_1	-	Antitoxin
EGA47_RS23745	70373..70603	-	231	WP_001736714.1	hypothetical protein	-
EGA47_RS23750	70767..71366	-	600	WP_032083981.1	hypothetical protein	-
EGA47_RS23755	71752..71952	-	201	WP_015059022.1	hypothetical protein	-
EGA47_RS23760	72084..72644	-	561	WP_000139328.1	fertility inhibition protein FinO	-
EGA47_RS23765	72699..73445	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / rmtB	-	1..87297	87297
	-	flank	IS/Tn	-	-	65758..66957	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T115274 WP_001312851.1 NZ_CP034108:c70068-69919 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T115274 NZ_CP034108:c70068-69919 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT115274 NZ_CP034108:70113-70172 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin