Detailed information of TA system TA115269

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22068..22494
• Replicon: plasmid pRCAD0514EC-2
• Accession: NZ_CP034108
• Organism: Escherichia coli strain RCAD0514

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EGA47_RS23450
• Protein ID: WP_001312861.1
• Coordinates: 22068..22226 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22270..22494 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGA47_RS23415	17124..17351	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
EGA47_RS23420	17445..18131	-	687	WP_015059009.1	PAS domain-containing protein	-
EGA47_RS23425	18322..18705	-	384	WP_000124981.1	relaxosome protein TraM	-
EGA47_RS23430	18982..19629	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
EGA47_RS23435	19926..20747	-	822	WP_001234445.1	DUF945 domain-containing protein	-
EGA47_RS23440	20858..21154	-	297	WP_001272251.1	hypothetical protein	-
EGA47_RS23445	21453..21830	+	378	Protein_33	hypothetical protein	-
EGA47_RS23450	22068..22226	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	22270..22494	-	225	NuclAT_0	-	Antitoxin
-	22270..22494	-	225	NuclAT_0	-	Antitoxin
-	22270..22494	-	225	NuclAT_0	-	Antitoxin
-	22270..22494	-	225	NuclAT_0	-	Antitoxin
EGA47_RS23455	22306..22485	+	180	WP_001309233.1	hypothetical protein	-
EGA47_RS23460	22506..23225	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
EGA47_RS23465	23222..23656	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EGA47_RS23470	23725..25748	-	2024	Protein_38	ParB/RepB/Spo0J family partition protein	-
EGA47_RS23475	25809..26042	-	234	WP_000006003.1	DUF905 family protein	-
EGA47_RS23480	26100..26627	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / blaTEM-1B / rmtB	-	1..87297	87297

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T115269 WP_001312861.1 NZ_CP034108:c22226-22068 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T115269 NZ_CP034108:c22226-22068 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT115269 NZ_CP034108:c22494-22270 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin