Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2236041..2236543 Replicon chromosome
Accession NZ_CP034106
Organism Escherichia coli strain RCAD0514

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EGA47_RS10545 Protein ID WP_000767829.1
Coordinates 2236289..2236543 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EGA47_RS10540 Protein ID WP_001259255.1
Coordinates 2236041..2236292 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGA47_RS10515 2231218..2232285 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EGA47_RS10520 2232285..2233355 - 1071 WP_053908449.1 histidinol-phosphate transaminase -
EGA47_RS10525 2233352..2234656 - 1305 WP_053908451.1 histidinol dehydrogenase -
EGA47_RS10530 2234662..2235561 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
EGA47_RS10535 2235707..2235757 - 51 WP_001364200.1 his operon leader peptide -
EGA47_RS10540 2236041..2236292 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EGA47_RS10545 2236289..2236543 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EGA47_RS10550 2236626..2237450 + 825 WP_000754737.1 SDR family oxidoreductase -
EGA47_RS10555 2237496..2238424 + 929 Protein_2045 LysR family transcriptional regulator -
EGA47_RS10560 2238639..2238701 + 63 WP_010723108.1 membrane protein YoeI -
EGA47_RS10565 2238691..2240049 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EGA47_RS10570 2240228..2241286 + 1059 WP_000492340.1 transport protein YeeE -
EGA47_RS10575 2241300..2241527 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T115252 WP_000767829.1 NZ_CP034106:2236289-2236543 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T115252 NZ_CP034106:2236289-2236543 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT115252 WP_001259255.1 NZ_CP034106:2236041-2236292 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT115252 NZ_CP034106:2236041-2236292 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References