Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 1127370..1128063 Replicon chromosome
Accession NZ_CP034106
Organism Escherichia coli strain RCAD0514

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag EGA47_RS05315 Protein ID WP_000415584.1
Coordinates 1127370..1127666 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag EGA47_RS05320 Protein ID WP_000650107.1
Coordinates 1127668..1128063 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGA47_RS05280 1122458..1122772 - 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -
EGA47_RS05285 1122803..1123384 - 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
EGA47_RS05290 1123703..1124035 + 333 WP_000917684.1 DUF2645 family protein -
EGA47_RS05295 1124081..1125430 - 1350 WP_001618857.1 two-component system sensor histidine kinase QseC -
EGA47_RS05300 1125427..1126086 - 660 WP_001221493.1 two-component system response regulator QseB -
EGA47_RS05305 1126238..1126630 + 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
EGA47_RS05310 1126683..1127165 + 483 WP_000183505.1 transcriptional regulator -
EGA47_RS05315 1127370..1127666 + 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
EGA47_RS05320 1127668..1128063 + 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
EGA47_RS05325 1128196..1129803 + 1608 WP_001295629.1 ABC transporter substrate-binding protein -
EGA47_RS05330 1129941..1132199 + 2259 WP_049253102.1 DNA topoisomerase IV subunit A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T115248 WP_000415584.1 NZ_CP034106:1127370-1127666 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T115248 NZ_CP034106:1127370-1127666 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT115248 WP_000650107.1 NZ_CP034106:1127668-1128063 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT115248 NZ_CP034106:1127668-1128063 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References