Detailed information of TA system TA115221

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF3/-
• Location: 380456..380964
• Replicon: chromosome
• Accession: NZ_CP034102
• Organism: Staphylococcus aureus strain O267

Toxin (Protein)

• Gene name: holin
• Uniprot ID: -
• Locus tag: SaO267_RS01905
• Protein ID: WP_000448764.1
• Coordinates: 380662..380964 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	380456..380566 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO267_RS01865	375550..375840	+	291	WP_000179860.1	hypothetical protein	-
SaO267_RS01870	375856..377766	+	1911	WP_000429563.1	hypothetical protein	-
SaO267_RS01875	377766..379232	+	1467	WP_000067157.1	BppU family phage baseplate upper protein	-
SaO267_RS01880	379232..379621	+	390	WP_001166604.1	DUF2977 domain-containing protein	-
SaO267_RS01885	379614..379778	+	165	WP_031807869.1	XkdX family protein	-
SaO267_RS01890	379824..380123	+	300	WP_000466773.1	DUF2951 family protein	-
SaO267_RS15090	380275..380464	+	190	Protein_359	putative holin-like toxin	-
-	380456..380566	-	111	NuclAT_0	-	Antitoxin
-	380456..380566	-	111	NuclAT_0	-	Antitoxin
-	380456..380566	-	111	NuclAT_0	-	Antitoxin
-	380456..380566	-	111	NuclAT_0	-	Antitoxin
SaO267_RS01900	380511..380611	-	101	Protein_360	hypothetical protein	-
SaO267_RS01905	380662..380964	+	303	WP_000448764.1	phage holin	Toxin
SaO267_RS01910	380976..382430	+	1455	WP_126116961.1	N-acetylmuramoyl-L-alanine amidase	-
SaO267_RS01920	383944..384441	+	498	WP_001803960.1	hypothetical protein	-
SaO267_RS01930	385001..385828	-	828	WP_000136028.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	320253..382430	62177

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (3-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11230.99 Da
• Isoelectric Point: 9.6587

>T115221 WP_000448764.1 NZ_CP034102:380662-380964 [Staphylococcus aureus]
METKVITRYIVLILALVNQFLANKGISPIPVDEESISSIILTVIALYTAYKDNPTSQEGRWANQKLKKYKAENKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T115221 NZ_CP034102:380662-380964 [Staphylococcus aureus]
ATGGAAACAAAAGTAATAACAAGATATATTGTATTAATTTTAGCGTTAGTCAATCAGTTTTTAGCGAACAAAGGTATAAG
CCCGATACCAGTAGATGAAGAGAGTATTTCATCGATTATCTTAACAGTCATTGCTTTATACACCGCTTATAAAGACAATC
CAACATCACAAGAAGGACGCTGGGCGAATCAAAAACTAAAGAAATATAAAGCTGAAAATAAGTATAGAAAAGCAACAGGA
CAAGCGCCAATTAAAGAAGTAATGACACCTACAAATATGAATGACACAAATGATTTAGGGTAG

Antitoxin

Nucleotide

• Length: 111 bp

>AT115221 NZ_CP034102:c380566-380456 [Staphylococcus aureus]
AAACATGTTACCCTAGTGAGCCCGTTAAAAAGACGGTGACCTCTGTAGTTAAATGAATTTATATAATCCTCTAACCATCG
TACTCGTCAAAGTTAGCGATGGTTATTTTTT

Structures

Antitoxin