Detailed information of TA system TA115177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 57413..58140
• Replicon: plasmid pR210-2-vir
• Accession: NZ_CP034083
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain R210-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: EHV09_RS27175
• Protein ID: WP_011251285.1
• Coordinates: 57829..58140 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EHV09_RS27170
• Protein ID: WP_011251286.1
• Coordinates: 57413..57832 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHV09_RS27135	52581..53051	-	471	WP_048333570.1	hypothetical protein	-
EHV09_RS31040	53244..53999	-	756	Protein_49	hypothetical protein	-
EHV09_RS27145	54595..55038	+	444	WP_048333569.1	hypothetical protein	-
EHV09_RS27150	55059..55847	+	789	WP_040217257.1	hypothetical protein	-
EHV09_RS27155	55861..56196	+	336	Protein_52	conjugal transfer protein TraI	-
EHV09_RS27160	56298..57266	-	969	WP_074428168.1	IS5 family transposase	-
EHV09_RS27170	57413..57832	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
EHV09_RS27175	57829..58140	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
EHV09_RS27180	58345..58803	-	459	Protein_56	DDE-type integrase/transposase/recombinase	-
EHV09_RS27185	58917..59614	+	698	Protein_57	IS1 family transposase	-
EHV09_RS27190	59631..60065	+	435	Protein_58	thioredoxin fold domain-containing protein	-
EHV09_RS27195	60082..60393	+	312	WP_011251282.1	hypothetical protein	-
EHV09_RS27200	60407..60748	+	342	WP_011251281.1	hypothetical protein	-
EHV09_RS27205	60808..61764	+	957	WP_011251280.1	DsbA family protein	-
EHV09_RS27210	61881..62456	+	576	WP_074424927.1	hypothetical protein	-
EHV09_RS27215	62564..63079	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..229648	229648
	-	inside	IScluster/Tn	-	-	47292..59614	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T115177 WP_011251285.1 NZ_CP034083:c58140-57829 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T115177 NZ_CP034083:c58140-57829 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT115177 WP_011251286.1 NZ_CP034083:c57832-57413 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT115177 NZ_CP034083:c57832-57413 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1