Detailed information of TA system TA115103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2869234..2869961
• Replicon: chromosome
• Accession: NZ_CP034067
• Organism: Shigella sonnei strain FDAARGOS_524

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: EGX92_RS14905
• Protein ID: WP_000547563.1
• Coordinates: 2869234..2869545 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGX92_RS14910
• Protein ID: WP_000126296.1
• Coordinates: 2869542..2869961 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX92_RS14875 (2864388)	2864388..2866085	+	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-
EGX92_RS14880 (2866095)	2866095..2866637	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
EGX92_RS14885 (2866637)	2866637..2867404	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
EGX92_RS14890 (2867401)	2867401..2867811	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
EGX92_RS14895 (2867804)	2867804..2868274	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EGX92_RS14900 (2868299)	2868299..2869072	+	774	WP_001026459.1	hypothetical protein	-
EGX92_RS14905 (2869234)	2869234..2869545	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
EGX92_RS14910 (2869542)	2869542..2869961	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
EGX92_RS14915 (2870075)	2870075..2871499	-	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
EGX92_RS14920 (2871508)	2871508..2872965	-	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EGX92_RS14925 (2873225)	2873225..2874235	+	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
EGX92_RS14930 (2874383)	2874383..2874910	+	528	WP_001078766.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T115103 WP_000547563.1 NZ_CP034067:2869234-2869545 [Shigella sonnei]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T115103 NZ_CP034067:2869234-2869545 [Shigella sonnei]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT115103 WP_000126296.1 NZ_CP034067:2869542-2869961 [Shigella sonnei]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT115103 NZ_CP034067:2869542-2869961 [Shigella sonnei]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22