Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 748881..749422 Replicon chromosome
Accession NZ_CP034067
Organism Shigella sonnei strain FDAARGOS_524

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EGX92_RS04380 Protein ID WP_000615976.1
Coordinates 749144..749422 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EGX92_RS04375 Protein ID WP_000729704.1
Coordinates 748881..749141 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX92_RS04350 (744350) 744350..746062 + 1713 Protein_710 flagellar biosynthesis protein FlhA -
EGX92_RS04355 (746185) 746185..746682 - 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
EGX92_RS04360 (746753) 746753..746881 - 129 Protein_712 ISAs1 family transposase -
EGX92_RS04365 (746936) 746936..747633 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
EGX92_RS04370 (747922) 747922..748695 - 774 WP_127148796.1 C40 family peptidase -
EGX92_RS04375 (748881) 748881..749141 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EGX92_RS04380 (749144) 749144..749422 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EGX92_RS04385 (749578) 749578..750318 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
EGX92_RS04390 (750289) 750289..751056 - 768 WP_000333380.1 class II glutamine amidotransferase -
EGX92_RS04395 (751262) 751262..751840 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T115098 WP_000615976.1 NZ_CP034067:749144-749422 [Shigella sonnei]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T115098 NZ_CP034067:749144-749422 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT115098 WP_000729704.1 NZ_CP034067:748881-749141 [Shigella sonnei]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT115098 NZ_CP034067:748881-749141 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References