Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3946947..3947641 Replicon chromosome
Accession NZ_CP034060
Organism Shigella flexneri strain FDAARGOS_535

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag EGX88_RS21900 Protein ID WP_001263491.1
Coordinates 3947243..3947641 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag EGX88_RS21895 Protein ID WP_000554759.1
Coordinates 3946947..3947240 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX88_RS21875 (3942586) 3942586..3943083 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
EGX88_RS21880 (3943300) 3943300..3945012 - 1713 Protein_3898 flagellar biosynthesis protein FlhA -
EGX88_RS21885 (3944984) 3944984..3945769 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
EGX88_RS21890 (3945840) 3945840..3946895 + 1056 WP_001226172.1 DNA polymerase IV -
EGX88_RS21895 (3946947) 3946947..3947240 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EGX88_RS21900 (3947243) 3947243..3947641 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EGX88_RS21905 (3947651) 3947651..3948103 + 453 WP_001059846.1 GNAT family N-acetyltransferase -
EGX88_RS21910 (3948349) 3948349..3949392 + 1044 WP_005060486.1 RNA ligase RtcB family protein -
EGX88_RS21915 (3949508) 3949508..3950068 + 561 Protein_3905 peptide chain release factor H -
EGX88_RS21920 (3950125) 3950125..3951582 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
EGX88_RS21925 (3951844) 3951844..3952302 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T115092 WP_001263491.1 NZ_CP034060:3947243-3947641 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T115092 NZ_CP034060:3947243-3947641 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT115092 WP_000554759.1 NZ_CP034060:3946947-3947240 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT115092 NZ_CP034060:3946947-3947240 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References