Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3847332..3848176 Replicon chromosome
Accession NZ_CP034060
Organism Shigella flexneri strain FDAARGOS_535

Toxin (Protein)


Gene name itaT Uniprot ID -
Locus tag EGX88_RS21385 Protein ID WP_014532172.1
Coordinates 3847715..3848176 (+) Length 154 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A5H0K0
Locus tag EGX88_RS21375 Protein ID WP_005053053.1
Coordinates 3847332..3847643 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX88_RS21350 (3842802) 3842802..3843299 + 498 Protein_3791 COX aromatic rich motif-containing protein -
EGX88_RS21355 (3843321) 3843321..3845312 + 1992 Protein_3792 cytochrome o ubiquinol oxidase subunit I -
EGX88_RS21360 (3845302) 3845302..3845916 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
EGX88_RS21365 (3845916) 3845916..3846245 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
EGX88_RS21370 (3846257) 3846257..3847147 + 891 WP_000971356.1 heme o synthase -
EGX88_RS21375 (3847332) 3847332..3847643 + 312 WP_005053053.1 DUF1778 domain-containing protein Antitoxin
EGX88_RS21385 (3847715) 3847715..3848176 + 462 WP_014532172.1 GNAT family N-acetyltransferase Toxin
EGX88_RS21395 (3849072) 3849072..3851225 + 2154 WP_005060811.1 SEL1-like repeat protein -
EGX88_RS21400 (3851278) 3851278..3853008 + 1731 WP_000645011.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3848224..3848727 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-133)

Antitoxin

Vibrio phage ICP1, Orf50

(15-97)


Sequences

Toxin        


Download         Length: 154 a.a.        Molecular weight: 16623.52 Da        Isoelectric Point: 9.9538

>T115091 WP_014532172.1 NZ_CP034060:3847715-3848176 [Shigella flexneri]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Download         Length: 462 bp

>T115091 NZ_CP034060:3847715-3848176 [Shigella flexneri]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11394.07 Da        Isoelectric Point: 10.3388

>AT115091 WP_005053053.1 NZ_CP034060:3847332-3847643 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT115091 NZ_CP034060:3847332-3847643 [Shigella flexneri]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A5H0K0

References