Detailed information of TA system TA115091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3847332..3848176
• Replicon: chromosome
• Accession: NZ_CP034060
• Organism: Shigella flexneri strain FDAARGOS_535

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: EGX88_RS21385
• Protein ID: WP_014532172.1
• Coordinates: 3847715..3848176 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: A5H0K0
• Locus tag: EGX88_RS21375
• Protein ID: WP_005053053.1
• Coordinates: 3847332..3847643 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX88_RS21350 (3842802)	3842802..3843299	+	498	Protein_3791	COX aromatic rich motif-containing protein	-
EGX88_RS21355 (3843321)	3843321..3845312	+	1992	Protein_3792	cytochrome o ubiquinol oxidase subunit I	-
EGX88_RS21360 (3845302)	3845302..3845916	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
EGX88_RS21365 (3845916)	3845916..3846245	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
EGX88_RS21370 (3846257)	3846257..3847147	+	891	WP_000971356.1	heme o synthase	-
EGX88_RS21375 (3847332)	3847332..3847643	+	312	WP_005053053.1	DUF1778 domain-containing protein	Antitoxin
EGX88_RS21385 (3847715)	3847715..3848176	+	462	WP_014532172.1	GNAT family N-acetyltransferase	Toxin
EGX88_RS21395 (3849072)	3849072..3851225	+	2154	WP_005060811.1	SEL1-like repeat protein	-
EGX88_RS21400 (3851278)	3851278..3853008	+	1731	WP_000645011.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP037923 Shigella flexneri 5a str. M90T 4168367..4168870 0.009	flank	IS/Tn	-	-	3848224..3848727	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (72-133)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-97)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16623.52 Da
• Isoelectric Point: 9.9538

>T115091 WP_014532172.1 NZ_CP034060:3847715-3848176 [Shigella flexneri]
MIAVTLLSINLYALRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVMLGGAR
KYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFYARLGFVQLSATPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 462 bp

>T115091 NZ_CP034060:3847715-3848176 [Shigella flexneri]
TTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACTG
TGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGAC
AACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGGAGCGCGG
AAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAAAAATTCACCCGGCATTACC
AATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCGA
CACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11394.07 Da
• Isoelectric Point: 10.3388

>AT115091 WP_005053053.1 NZ_CP034060:3847332-3847643 [Shigella flexneri]
MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT115091 NZ_CP034060:3847332-3847643 [Shigella flexneri]
ATGCCTGGAAAAACAGCCGCTCTATACGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A5H0K0