Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3810376..3810994 Replicon chromosome
Accession NZ_CP034060
Organism Shigella flexneri strain FDAARGOS_535

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EGX88_RS21180 Protein ID WP_001291435.1
Coordinates 3810776..3810994 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag EGX88_RS21175 Protein ID WP_000344797.1
Coordinates 3810376..3810750 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGX88_RS21165 (3805465) 3805465..3806658 + 1194 WP_024260158.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EGX88_RS21170 (3806681) 3806681..3809830 + 3150 WP_001132485.1 efflux RND transporter permease AcrB -
EGX88_RS21175 (3810376) 3810376..3810750 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
EGX88_RS21180 (3810776) 3810776..3810994 + 219 WP_001291435.1 HHA domain-containing protein Toxin
EGX88_RS21185 (3811166) 3811166..3811717 + 552 WP_000102577.1 maltose O-acetyltransferase -
EGX88_RS21190 (3811833) 3811833..3812303 + 471 WP_000136192.1 YlaC family protein -
EGX88_RS21195 (3812467) 3812467..3814017 + 1551 WP_005106783.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EGX88_RS21200 (3814059) 3814059..3814412 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EGX88_RS21210 (3814791) 3814791..3815102 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3815132..3816460 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T115090 WP_001291435.1 NZ_CP034060:3810776-3810994 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T115090 NZ_CP034060:3810776-3810994 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT115090 WP_000344797.1 NZ_CP034060:3810376-3810750 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT115090 NZ_CP034060:3810376-3810750 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References