Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/Tad-HTH_37 |
| Location | 3508618..3509146 | Replicon | chromosome |
| Accession | NZ_CP034060 | ||
| Organism | Shigella flexneri strain FDAARGOS_535 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | - |
| Locus tag | EGX88_RS19555 | Protein ID | WP_005061113.1 |
| Coordinates | 3508618..3508848 (+) | Length | 77 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | Q32DW6 |
| Locus tag | EGX88_RS19560 | Protein ID | WP_000147081.1 |
| Coordinates | 3508838..3509146 (+) | Length | 103 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EGX88_RS19520 (3503686) | 3503686..3503886 | - | 201 | WP_000111769.1 | protein ninH | - |
| EGX88_RS19525 (3503981) | 3503981..3504238 | - | 258 | WP_001064799.1 | hypothetical protein | - |
| EGX88_RS19530 (3504235) | 3504235..3505626 | - | 1392 | WP_000211324.1 | replicative DNA helicase | - |
| EGX88_RS19535 (3505623) | 3505623..3506501 | - | 879 | WP_000988183.1 | ATP-binding protein | - |
| EGX88_RS19540 (3506512) | 3506512..3507420 | - | 909 | WP_001247840.1 | hypothetical protein | - |
| EGX88_RS19545 (3507407) | 3507407..3507640 | - | 234 | WP_000618004.1 | hypothetical protein | - |
| EGX88_RS26325 (3507637) | 3507637..3508089 | - | 453 | WP_232046050.1 | Rha family transcriptional regulator | - |
| EGX88_RS26330 (3508147) | 3508147..3508605 | - | 459 | WP_000961311.1 | ash family protein | - |
| EGX88_RS19555 (3508618) | 3508618..3508848 | + | 231 | WP_005061113.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| EGX88_RS19560 (3508838) | 3508838..3509146 | + | 309 | WP_000147081.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| EGX88_RS19565 (3509188) | 3509188..3509364 | - | 177 | WP_001252556.1 | NinE family protein | - |
| EGX88_RS19570 (3509361) | 3509361..3509771 | - | 411 | WP_000814621.1 | recombination protein NinB | - |
| EGX88_RS19575 (3509768) | 3509768..3510046 | - | 279 | WP_005061104.1 | hypothetical protein | - |
| EGX88_RS19580 (3510121) | 3510121..3510315 | - | 195 | Protein_3462 | hypothetical protein | - |
| EGX88_RS19585 (3510385) | 3510385..3511553 | + | 1169 | WP_000747032.1 | IS3-like element IS911 family transposase | - |
| EGX88_RS19595 (3511683) | 3511683..3512812 | + | 1130 | WP_112361761.1 | IS3-like element ISSfl10 family transposase | - |
| EGX88_RS19600 (3512859) | 3512859..3512945 | - | 87 | Protein_3465 | class II holin family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | ipaH4.5 / ipaH9.8 | 3470857..3570383 | 99526 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 77 a.a. Molecular weight: 8665.12 Da Isoelectric Point: 10.5308
>T115089 WP_005061113.1 NZ_CP034060:3508618-3508848 [Shigella flexneri]
MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW
MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKAQKTPPAERKKAETRMKEVKHDW
Download Length: 231 bp
>T115089 NZ_CP034060:3508618-3508848 [Shigella flexneri]
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAG
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA
ATGAAGGTATACGGCAGCAATCTCGGAGAACCTCACACCAGCGCCTTTGGTGACGGGCTTTTCGAACTCAGAATTAAAGG
CAACGATGGGATCGCGCGTGTTTTTTACTGCACTCTGACAGGGAAACGCATCATCATGCTGCATAGTTTTGTAAAGAAAG
CGCAGAAAACACCGCCAGCCGAACGCAAGAAAGCTGAAACCAGAATGAAGGAGGTTAAGCATGACTGGTAA
Antitoxin
Download Length: 103 a.a. Molecular weight: 11185.94 Da Isoelectric Point: 8.5840
>AT115089 WP_000147081.1 NZ_CP034060:3508838-3509146 [Shigella flexneri]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV
Download Length: 309 bp
>AT115089 NZ_CP034060:3508838-3509146 [Shigella flexneri]
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA
ATGACTGGTAAACGCACTCTCCCCACCATGACACACGACGAAATGGCAGCCAAATGGATGGAAGACCCGGCTTTTAAAGC
AGAATACGACGCTATCGCTGACGAATTCGCACTGCTTGATGAAATGCTGGCAGCACGCAAAGAAGCTGGCTTAACTCAGG
CTGAAGTTGCCGAGCGAATGGGAACAAAAGCGACCGCGATCACCAGAATGGAAAGTAATCTCGCATCAGGTATCAGCGGC
CCATCATTTGCCACACTAAAAAAATTCGCCCGCGCTACTGGAAAAAAACTCCAGATCCGCTTCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|