Detailed information of TA system TA115086

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2758611..2758836
• Replicon: chromosome
• Accession: NZ_CP034060
• Organism: Shigella flexneri strain FDAARGOS_535

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: EGX88_RS15530
• Protein ID: WP_000813254.1
• Coordinates: 2758611..2758766 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2758778..2758836 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX88_RS15490	2753894..2754262	-	369	WP_011069357.1	hypothetical protein	-
EGX88_RS15500	2755508..2755681	-	174	WP_000504450.1	hypothetical protein	-
EGX88_RS15505	2755834..2756388	-	555	WP_000640143.1	DUF1133 family protein	-
EGX88_RS15510	2756385..2756675	-	291	WP_000228038.1	DUF1364 domain-containing protein	-
EGX88_RS15515	2756675..2757274	-	600	WP_000940329.1	DUF1367 family protein	-
EGX88_RS15520	2757408..2758105	+	698	WP_225620329.1	IS1 family transposase	-
EGX88_RS15530	2758611..2758766	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2758778..2758836	+	59	-	-	Antitoxin
EGX88_RS15535	2759221..2759586	-	366	WP_000610655.1	DUF551 domain-containing protein	-
EGX88_RS15540	2759586..2760251	-	666	WP_000208062.1	hypothetical protein	-
EGX88_RS15545	2760248..2760613	-	366	WP_001229298.1	HNH endonuclease signature motif containing protein	-
EGX88_RS15550	2760615..2760833	-	219	WP_000256998.1	DUF4014 family protein	-
EGX88_RS15555	2760926..2761282	-	357	WP_005048249.1	hypothetical protein	-
EGX88_RS15560	2761340..2761762	-	423	WP_001118167.1	DUF977 family protein	-
EGX88_RS15565	2761777..2762520	-	744	WP_000788999.1	ATP-binding protein	-
EGX88_RS26200	2762666..2762835	-	170	Protein_2688	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	sitABCD	ipaH9.8	2712335..2768002	55667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T115086 WP_000813254.1 NZ_CP034060:c2758766-2758611 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T115086 NZ_CP034060:c2758766-2758611 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT115086 NZ_CP034060:2758778-2758836 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATTTGTGAGACATAGATTGGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin