Detailed information of TA system TA115075

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2111332..2111557
• Replicon: chromosome
• Accession: NZ_CP034060
• Organism: Shigella flexneri strain FDAARGOS_535

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: EGX88_RS11915
• Protein ID: WP_000813254.1
• Coordinates: 2111402..2111557 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2111332..2111390 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX88_RS11870	2106519..2107781	-	1263	Protein_1987	tyrosine-type recombinase/integrase	-
EGX88_RS11880	2108119..2108916	-	798	WP_024260703.1	DgsA anti-repressor MtfA	-
EGX88_RS11890	2109127..2110167	-	1041	WP_005096324.1	tyrosine-type recombinase/integrase	-
EGX88_RS11895	2110167..2110307	-	141	Protein_1990	DUF4224 domain-containing protein	-
EGX88_RS11900	2110334..2110750	+	417	WP_005069274.1	hypothetical protein	-
-	2111332..2111390	-	59	-	-	Antitoxin
EGX88_RS11915	2111402..2111557	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
EGX88_RS11925	2111725..2112003	+	279	WP_011069426.1	hypothetical protein	-
EGX88_RS11930	2112005..2113063	+	1059	WP_001265248.1	DUF968 domain-containing protein	-
EGX88_RS11935	2113064..2113429	+	366	WP_000140017.1	RusA family crossover junction endodeoxyribonuclease	-
EGX88_RS11940	2113426..2114114	+	689	Protein_1996	bacteriophage antitermination protein Q	-
EGX88_RS11970	2114910..2115125	+	216	WP_000839572.1	class II holin family protein	-
EGX88_RS11975	2115224..2115898	+	675	WP_004967157.1	IS66-like element accessory protein TnpA	-
EGX88_RS11980	2115895..2116245	+	351	WP_005049241.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	ipaH9.8	2070175..2144856	74681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T115075 WP_000813254.1 NZ_CP034060:2111402-2111557 [Shigella flexneri]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T115075 NZ_CP034060:2111402-2111557 [Shigella flexneri]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT115075 NZ_CP034060:c2111390-2111332 [Shigella flexneri]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin