Detailed information of TA system TA115062

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23327..23753
• Replicon: plasmid unnamed3
• Accession: NZ_CP034048
• Organism: Klebsiella pneumoniae strain KP_NORM_BLD_2014_104014

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EHF42_RS30650
• Protein ID: WP_001312861.1
• Coordinates: 23327..23485 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	23529..23753 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHF42_RS28850	18900..19604	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
EHF42_RS28855	19651..19872	-	222	WP_124737165.1	hypothetical protein	-
EHF42_RS30755	20264..20468	+	205	Protein_28	pilus protein	-
EHF42_RS28875	20553..22166	-	1614	WP_000080200.1	IS66-like element ISEc23 family transposase	-
EHF42_RS28880	22197..22547	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
EHF42_RS28885	22544..22969	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
EHF42_RS30650	23327..23485	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	23529..23753	-	225	NuclAT_0	-	Antitoxin
-	23529..23753	-	225	NuclAT_0	-	Antitoxin
-	23529..23753	-	225	NuclAT_0	-	Antitoxin
-	23529..23753	-	225	NuclAT_0	-	Antitoxin
EHF42_RS28895	23765..24484	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
EHF42_RS28900	24481..24915	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EHF42_RS28905	24984..26952	-	1969	Protein_35	ParB/RepB/Spo0J family partition protein	-
EHF42_RS28910	27013..27246	-	234	WP_000006003.1	DUF905 family protein	-
EHF42_RS28915	27304..27831	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / blaOXA-1 / aac(6')-Ib-cr	-	1..76757	76757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T115062 WP_001312861.1 NZ_CP034048:c23485-23327 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T115062 NZ_CP034048:c23485-23327 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT115062 NZ_CP034048:c23753-23529 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin