Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3900609..3901206 Replicon chromosome
Accession NZ_CP034045
Organism Klebsiella pneumoniae strain KP_NORM_BLD_2014_104014

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag EHF42_RS19295 Protein ID WP_004142563.1
Coordinates 3900889..3901206 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag EHF42_RS19290 Protein ID WP_004142561.1
Coordinates 3900609..3900896 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHF42_RS19260 3896689..3896937 + 249 WP_020325243.1 DUF1158 domain-containing protein -
EHF42_RS19265 3896955..3897296 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
EHF42_RS19270 3897327..3898442 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
EHF42_RS19275 3898622..3899203 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
EHF42_RS19280 3899203..3899571 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
EHF42_RS19285 3899691..3900344 + 654 WP_117040415.1 oxygen-insensitive NAD(P)H nitroreductase -
EHF42_RS19290 3900609..3900896 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
EHF42_RS19295 3900889..3901206 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EHF42_RS19300 3901391..3902434 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
EHF42_RS19310 3903104..3903970 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
EHF42_RS19315 3904079..3905506 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T115053 WP_004142563.1 NZ_CP034045:c3901206-3900889 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T115053 NZ_CP034045:c3901206-3900889 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT115053 WP_004142561.1 NZ_CP034045:c3900896-3900609 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT115053 NZ_CP034045:c3900896-3900609 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References