Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 362310..362956 Replicon chromosome
Accession NZ_CP034039
Organism Klebsiella pneumoniae subsp. pneumoniae strain CRK0298

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag C5X33_RS01700 Protein ID WP_004174016.1
Coordinates 362310..362657 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4S7L580
Locus tag C5X33_RS01705 Protein ID WP_019725405.1
Coordinates 362657..362956 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5X33_RS01690 358236..359669 + 1434 WP_002920564.1 glycogen synthase GlgA -
C5X33_RS01695 359687..362134 + 2448 WP_002920561.1 glycogen phosphorylase -
C5X33_RS01700 362310..362657 + 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C5X33_RS01705 362657..362956 + 300 WP_019725405.1 XRE family transcriptional regulator Antitoxin
C5X33_RS01710 363019..364527 - 1509 WP_019704656.1 glycerol-3-phosphate dehydrogenase -
C5X33_RS01715 364732..365061 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
C5X33_RS01720 365112..365942 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
C5X33_RS01725 365992..366750 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T115022 WP_004174016.1 NZ_CP034039:362310-362657 [Klebsiella pneumoniae subsp. pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T115022 NZ_CP034039:362310-362657 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10775.53 Da        Isoelectric Point: 9.7681

>AT115022 WP_019725405.1 NZ_CP034039:362657-362956 [Klebsiella pneumoniae subsp. pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKSYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT115022 NZ_CP034039:362657-362956 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A4S7L580

References