Detailed information of TA system TA114733

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 56500..56764
• Replicon: plasmid p4_L382
• Accession: NZ_CP033963
• Organism: Klebsiella pneumoniae strain L482

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: EHC63_RS29665
• Protein ID: WP_001303307.1
• Coordinates: 56612..56764 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	56500..56562 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC63_RS29650	52602..53672	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
EHC63_RS29655	53691..54899	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	55079..55139	-	61	NuclAT_1	-	-
-	55079..55139	-	61	NuclAT_1	-	-
-	55079..55139	-	61	NuclAT_1	-	-
-	55079..55139	-	61	NuclAT_1	-	-
EHC63_RS29660	55206..56297	-	1092	WP_000426061.1	hypothetical protein	-
-	56500..56562	-	63	NuclAT_0	-	Antitoxin
-	56500..56562	-	63	NuclAT_0	-	Antitoxin
-	56500..56562	-	63	NuclAT_0	-	Antitoxin
-	56500..56562	-	63	NuclAT_0	-	Antitoxin
EHC63_RS29665	56612..56764	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
EHC63_RS29670	56836..57087	-	252	WP_001291968.1	hypothetical protein	-
-	57474..57525	-	52	NuclAT_2	-	-
-	57474..57525	-	52	NuclAT_2	-	-
-	57474..57525	-	52	NuclAT_2	-	-
-	57474..57525	-	52	NuclAT_2	-	-
EHC63_RS30735	58011..58187	-	177	WP_001054900.1	hypothetical protein	-
EHC63_RS29685	58396..58605	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
EHC63_RS29690	58703..59317	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
EHC63_RS29695	59393..61561	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..102879	102879

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T114733 WP_001303307.1 NZ_CP033963:56612-56764 [Klebsiella pneumoniae]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T114733 NZ_CP033963:56612-56764 [Klebsiella pneumoniae]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT114733 NZ_CP033963:c56562-56500 [Klebsiella pneumoniae]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin