Detailed information of TA system TA114731

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 135063..135316
• Replicon: plasmid p3_L382
• Accession: NZ_CP033962
• Organism: Klebsiella pneumoniae strain L482

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: EHC63_RS29240
• Protein ID: WP_001312851.1
• Coordinates: 135167..135316 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	135063..135122 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC63_RS29195	130723..130788	-	66	Protein_178	helix-turn-helix domain-containing protein	-
EHC63_RS29200	130841..131545	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
EHC63_RS29205	131609..131770	+	162	Protein_180	DNA helicase	-
EHC63_RS29210	131790..132536	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
EHC63_RS29215	132591..133151	+	561	WP_000139328.1	fertility inhibition protein FinO	-
EHC63_RS29220	133283..133483	+	201	WP_015059022.1	hypothetical protein	-
EHC63_RS29225	133869..134468	+	600	WP_032083981.1	hypothetical protein	-
EHC63_RS29230	134632..134862	+	231	WP_001736714.1	hypothetical protein	-
-	135063..135122	-	60	NuclAT_1	-	Antitoxin
-	135063..135122	-	60	NuclAT_1	-	Antitoxin
-	135063..135122	-	60	NuclAT_1	-	Antitoxin
-	135063..135122	-	60	NuclAT_1	-	Antitoxin
EHC63_RS29240	135167..135316	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
EHC63_RS29245	135600..135848	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / rmtB / blaTEM-1B / blaKPC-2 / catA2	-	1..136159	136159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T114731 WP_001312851.1 NZ_CP033962:135167-135316 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T114731 NZ_CP033962:135167-135316 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT114731 NZ_CP033962:c135122-135063 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin