Detailed information of TA system TA114727

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28394..28820
• Replicon: plasmid p3_L382
• Accession: NZ_CP033962
• Organism: Klebsiella pneumoniae strain L482

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EHC63_RS28390
• Protein ID: WP_001312861.1
• Coordinates: 28394..28552 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	28596..28820 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC63_RS28345	23449..23676	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
EHC63_RS28350	23770..24456	-	687	WP_015059009.1	PAS domain-containing protein	-
EHC63_RS28355	24647..25030	-	384	WP_000124981.1	relaxosome protein TraM	-
EHC63_RS28360	25307..25954	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
EHC63_RS28365	26251..27072	-	822	WP_001234445.1	DUF945 domain-containing protein	-
EHC63_RS28370	27183..27479	-	297	WP_001272251.1	hypothetical protein	-
EHC63_RS30695	27779..28156	+	378	Protein_41	hypothetical protein	-
EHC63_RS28390	28394..28552	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	28596..28820	-	225	NuclAT_0	-	Antitoxin
-	28596..28820	-	225	NuclAT_0	-	Antitoxin
-	28596..28820	-	225	NuclAT_0	-	Antitoxin
-	28596..28820	-	225	NuclAT_0	-	Antitoxin
EHC63_RS28395	28832..29551	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
EHC63_RS28400	29548..29982	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EHC63_RS28405	30051..32074	-	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
EHC63_RS28410	32135..32368	-	234	WP_000006003.1	DUF905 family protein	-
EHC63_RS28415	32426..32953	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / rmtB / blaTEM-1B / blaKPC-2 / catA2	-	1..136159	136159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T114727 WP_001312861.1 NZ_CP033962:c28552-28394 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T114727 NZ_CP033962:c28552-28394 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT114727 NZ_CP033962:c28820-28596 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin