Detailed information of TA system TA114725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 30101..30828
• Replicon: plasmid p2_L382
• Accession: NZ_CP033961
• Organism: Klebsiella pneumoniae strain L482

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: EHC63_RS27455
• Protein ID: WP_011251285.1
• Coordinates: 30517..30828 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EHC63_RS27450
• Protein ID: WP_011251286.1
• Coordinates: 30101..30520 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC63_RS30675	25386..26141	+	756	Protein_26	hypothetical protein	-
EHC63_RS27420	26334..26804	+	471	WP_048333570.1	hypothetical protein	-
EHC63_RS27425	26826..27794	+	969	WP_004225014.1	IS5 family transposase	-
EHC63_RS27430	27828..28535	+	708	WP_094819173.1	hypothetical protein	-
EHC63_RS27435	28549..28884	+	336	Protein_30	conjugal transfer protein TraI	-
EHC63_RS27440	28986..29954	-	969	WP_086642568.1	IS5 family transposase	-
EHC63_RS27450	30101..30520	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
EHC63_RS27455	30517..30828	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
EHC63_RS27460	31033..31491	-	459	Protein_34	DDE-type integrase/transposase/recombinase	-
EHC63_RS27465	31605..32302	+	698	Protein_35	IS1 family transposase	-
EHC63_RS27470	32319..32753	+	435	Protein_36	thioredoxin fold domain-containing protein	-
EHC63_RS27475	32770..33081	+	312	WP_011251282.1	hypothetical protein	-
EHC63_RS27480	33095..33436	+	342	WP_011251281.1	hypothetical protein	-
EHC63_RS27485	33496..34452	+	957	WP_011251280.1	DsbA family protein	-
EHC63_RS27490	34569..35144	+	576	WP_074424927.1	hypothetical protein	-
EHC63_RS27495	35252..35767	+	516	WP_094819131.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA	1..145683	145683
	-	inside	IScluster/Tn	-	-	23215..32302	9087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T114725 WP_011251285.1 NZ_CP033961:c30828-30517 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T114725 NZ_CP033961:c30828-30517 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT114725 WP_011251286.1 NZ_CP033961:c30520-30101 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT114725 NZ_CP033961:c30520-30101 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1