Detailed information of TA system TA114700

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35048..35318
• Replicon: plasmid p3_L39
• Accession: NZ_CP033956
• Organism: Klebsiella pneumoniae strain L39_2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EG820_RS28730
• Protein ID: WP_001312861.1
• Coordinates: 35160..35318 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35048..35111 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG820_RS28705	30759..31286	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
EG820_RS28710	31344..31577	+	234	WP_000006003.1	DUF905 family protein	-
EG820_RS28715	31638..33661	+	2024	Protein_42	ParB/RepB/Spo0J family partition protein	-
EG820_RS28720	33730..34164	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EG820_RS28725	34161..34880	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	34892..35116	+	225	NuclAT_0	-	-
-	34892..35116	+	225	NuclAT_0	-	-
-	34892..35116	+	225	NuclAT_0	-	-
-	34892..35116	+	225	NuclAT_0	-	-
-	35048..35111	-	64	-	-	Antitoxin
EG820_RS28730	35160..35318	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
EG820_RS30700	35556..35933	-	378	Protein_46	hypothetical protein	-
EG820_RS28750	36233..36529	+	297	WP_001272251.1	hypothetical protein	-
EG820_RS28755	36640..37461	+	822	WP_001234445.1	DUF945 domain-containing protein	-
EG820_RS28760	37758..38405	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
EG820_RS28765	38682..39065	+	384	WP_000124981.1	relaxosome protein TraM	-
EG820_RS28775	39346..40050	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..133772	133772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T114700 WP_001312861.1 NZ_CP033956:35160-35318 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T114700 NZ_CP033956:35160-35318 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT114700 NZ_CP033956:c35111-35048 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin