Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3901237..3901834 Replicon chromosome
Accession NZ_CP033946
Organism Klebsiella pneumoniae subsp. pneumoniae strain ARLG-3135

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag EG819_RS19445 Protein ID WP_004142563.1
Coordinates 3901517..3901834 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag EG819_RS19440 Protein ID WP_004142561.1
Coordinates 3901237..3901524 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EG819_RS19410 3897332..3897580 + 249 WP_002893037.1 DUF1158 domain-containing protein -
EG819_RS19415 3897598..3897939 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
EG819_RS19420 3897970..3899085 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
EG819_RS19425 3899265..3899849 + 585 WP_071579668.1 TetR/AcrR family transcriptional regulator -
EG819_RS19430 3899846..3900214 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
EG819_RS19435 3900334..3900972 + 639 WP_064277739.1 oxygen-insensitive NAD(P)H nitroreductase -
EG819_RS19440 3901237..3901524 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
EG819_RS19445 3901517..3901834 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EG819_RS19450 3902019..3903062 - 1044 WP_040153598.1 DUF2157 domain-containing protein -
EG819_RS19460 3903732..3904598 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
EG819_RS19465 3904707..3906134 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T114670 WP_004142563.1 NZ_CP033946:c3901834-3901517 [Klebsiella pneumoniae subsp. pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T114670 NZ_CP033946:c3901834-3901517 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT114670 WP_004142561.1 NZ_CP033946:c3901524-3901237 [Klebsiella pneumoniae subsp. pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT114670 NZ_CP033946:c3901524-3901237 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References