Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 32347..32883 Replicon plasmid pPSEM90-5
Accession NZ_CP033942
Organism Piscirickettsia salmonis strain EM-90

Toxin (Protein)


Gene name yoeB Uniprot ID A0A6I5YBD1
Locus tag EG881_RS17810 Protein ID WP_016211910.1
Coordinates 32611..32883 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag EG881_RS17805 Protein ID WP_032126795.1
Coordinates 32347..32607 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EG881_RS17770 28085..28411 - 327 WP_016212298.1 hypothetical protein -
EG881_RS17775 28652..29128 + 477 WP_051307374.1 Clp protease ClpP -
EG881_RS17780 29243..29536 + 294 WP_075275158.1 transposase -
EG881_RS17785 29497..30177 + 681 Protein_42 IS3 family transposase -
EG881_RS17790 30382..30684 + 303 WP_098082791.1 IS3 family transposase -
EG881_RS17795 30693..31394 - 702 WP_129556698.1 IS6 family transposase -
EG881_RS17800 31484..32074 - 591 WP_016211912.1 site-specific integrase -
EG881_RS17805 32347..32607 + 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EG881_RS17810 32611..32883 + 273 WP_016211910.1 Txe/YoeB family addiction module toxin Toxin
EG881_RS17815 33209..34330 - 1122 WP_016211913.1 hypothetical protein -
EG881_RS17820 34763..35161 + 399 WP_075273786.1 helix-turn-helix domain-containing protein -
EG881_RS17825 35170..35727 - 558 WP_081377915.1 IS3 family transposase -
EG881_RS17830 35872..36201 - 330 WP_081377914.1 IS3 family transposase -
EG881_RS17835 36318..36611 - 294 WP_032126637.1 transposase -
EG881_RS17840 36671..37273 + 603 Protein_53 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..167750 167750


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10739.22 Da        Isoelectric Point: 7.8926

>T114654 WP_016211910.1 NZ_CP033942:32611-32883 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRTPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
IAACRYHYDQ

Download         Length: 273 bp

>T114654 NZ_CP033942:32611-32883 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
ACTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCACGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTGCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT114654 WP_032126795.1 NZ_CP033942:32347-32607 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT114654 NZ_CP033942:32347-32607 [Piscirickettsia salmonis]
ATGTTACGAGACAACATTAATGCTACGGCAGCGCGGGATAATTTTAAGAGTACCTGTGATACGGTGTGTGAAAACAATGC
CCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGCATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I5YBD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References