114645

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 24103..24812 Replicon chromosome
Accession NZ_CP033911
Organism Mycobacterium avium subsp. paratuberculosis strain MAPK_JB16/15

Toxin (Protein)


Gene name HigB2 Uniprot ID X7V9P5
Locus tag EGM64_RS00130 Protein ID WP_003873387.1
Coordinates 24103..24507 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID Q73S10
Locus tag EGM64_RS00135 Protein ID WP_003873388.1
Coordinates 24504..24812 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGM64_RS00105 19417..20247 - 831 WP_003874399.1 hypothetical protein -
EGM64_RS00110 20645..20953 + 309 WP_003874400.1 WhiB family transcriptional regulator -
EGM64_RS00115 20996..21760 + 765 WP_003874401.1 superoxide dismutase family protein -
EGM64_RS00120 21917..23116 - 1200 WP_010950287.1 FAD-dependent oxidoreductase -
EGM64_RS00125 23171..23863 - 693 WP_010950286.1 DUF4352 domain-containing protein -
EGM64_RS00130 24103..24507 + 405 WP_003873387.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGM64_RS00135 24504..24812 + 309 WP_003873388.1 helix-turn-helix transcriptional regulator Antitoxin
EGM64_RS00145 25299..26825 - 1527 WP_003879517.1 adenylate/guanylate cyclase domain-containing protein -
EGM64_RS00150 26920..28536 - 1617 WP_003873390.1 chaperonin GroEL -
EGM64_RS00155 28625..28927 - 303 WP_003873391.1 co-chaperone GroES -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-109)

Antitoxin

HigA2-like, helix-turn-helix domain

(31-91)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15503.82 Da        Isoelectric Point: 10.3815

>T114645 WP_003873387.1 NZ_CP033911:24103-24507 [Mycobacterium avium subsp. paratuberculosis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Download         Length: 405 bp

>T114645 NZ_CP033911:24103-24507 [Mycobacterium avium subsp. paratuberculosis]
GTGCGGAAATGGGAGGTTGACCTGACTCTCATCGAAGCCTGGATGGATGCCCTCGATGACGAGGAGTACGACAACTTGAT
AGCAGCGCTCGAGCAGTTGGAGGAACACGGTCCAATTACCCGGCGGCCGTTTGTGGACACCCTTGAAGGGTCAAGGCACC
CGAACATGAAGGAGCTCCGTCCGCGCCCCACGAAAGCTGGAGCCCACATTCGCGTGCTATTCGCCTTCGATACCCGGTCG
CGGGCGATCATGTTGATCGCTGGAGACAAGGCGGGCAATTGGTCCAAGTGGTATGCCAAGCACATTCCCATTGCCGACGA
GCTCTTCGATGCTCACCAAAAGCGTTTACACAAGGCAGCAGCCAAAGCCACCAATCGGAAACCAAGGAAGGGAAAGAAAC
GATGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11445.94 Da        Isoelectric Point: 10.0801

>AT114645 WP_003873388.1 NZ_CP033911:24504-24812 [Mycobacterium avium subsp. paratuberculosis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAQYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Download         Length: 309 bp

>AT114645 NZ_CP033911:24504-24812 [Mycobacterium avium subsp. paratuberculosis]
ATGACCAATCTTGATGACATGCGCCGTCGCCGTCCCGGCAACCGGACCCGTATCGACGCGATCAAGGCGGAGATGGACCG
CGAGGTTGCCCAGTACCGTCTCCGCGAACTGCGCGAGGCTGCCGGCTACACGCAGACGACTCTCGCTGCGGCTATTGGCG
TCGGGCAGAACCGCGTCTCGCAGATGGAACATGGCGATCTGGGCACGAGCCGGGTCGACACTCTCCGCAAGTACGTGGAA
GCGACTGGGGGTGAGCTGGAAGTGTCCGTCAAGCGTCCGGACGGGTCACGGGTTCTCCTCAGCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X7V9P5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4U5M0

References