Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1904139..1904848 Replicon chromosome
Accession NZ_CP033910
Organism Mycobacterium avium subsp. paratuberculosis strain MAPK_CN4/13

Toxin (Protein)


Gene name HigB2 Uniprot ID X7V9P5
Locus tag EGM63_RS09475 Protein ID WP_003873387.1
Coordinates 1904444..1904848 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID Q73S10
Locus tag EGM63_RS09470 Protein ID WP_003873388.1
Coordinates 1904139..1904447 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGM63_RS09450 1900024..1900326 + 303 WP_003873391.1 co-chaperone GroES -
EGM63_RS09455 1900415..1902031 + 1617 WP_003873390.1 chaperonin GroEL -
EGM63_RS09460 1902126..1903652 + 1527 WP_003879517.1 adenylate/guanylate cyclase domain-containing protein -
EGM63_RS09470 1904139..1904447 - 309 WP_003873388.1 helix-turn-helix transcriptional regulator Antitoxin
EGM63_RS09475 1904444..1904848 - 405 WP_003873387.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGM63_RS09480 1905088..1905780 + 693 WP_010950286.1 DUF4352 domain-containing protein -
EGM63_RS09485 1905835..1907034 + 1200 WP_010950287.1 FAD-dependent oxidoreductase -
EGM63_RS09490 1907191..1907955 - 765 WP_003874401.1 superoxide dismutase family protein -
EGM63_RS09495 1907998..1908306 - 309 WP_003874400.1 WhiB family transcriptional regulator -
EGM63_RS09500 1908704..1909534 + 831 WP_003874399.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-109)

Antitoxin

HigA2-like, helix-turn-helix domain

(31-91)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15503.82 Da        Isoelectric Point: 10.3815

>T114641 WP_003873387.1 NZ_CP033910:c1904848-1904444 [Mycobacterium avium subsp. paratuberculosis]
VRKWEVDLTLIEAWMDALDDEEYDNLIAALEQLEEHGPITRRPFVDTLEGSRHPNMKELRPRPTKAGAHIRVLFAFDTRS
RAIMLIAGDKAGNWSKWYAKHIPIADELFDAHQKRLHKAAAKATNRKPRKGKKR

Download         Length: 405 bp

>T114641 NZ_CP033910:c1904848-1904444 [Mycobacterium avium subsp. paratuberculosis]
GTGCGGAAATGGGAGGTTGACCTGACTCTCATCGAAGCCTGGATGGATGCCCTCGATGACGAGGAGTACGACAACTTGAT
AGCAGCGCTCGAGCAGTTGGAGGAACACGGTCCAATTACCCGGCGGCCGTTTGTGGACACCCTTGAAGGGTCAAGGCACC
CGAACATGAAGGAGCTCCGTCCGCGCCCCACGAAAGCTGGAGCCCACATTCGCGTGCTATTCGCCTTCGATACCCGGTCG
CGGGCGATCATGTTGATCGCTGGAGACAAGGCGGGCAATTGGTCCAAGTGGTATGCCAAGCACATTCCCATTGCCGACGA
GCTCTTCGATGCTCACCAAAAGCGTTTACACAAGGCAGCAGCCAAAGCCACCAATCGGAAACCAAGGAAGGGAAAGAAAC
GATGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11445.94 Da        Isoelectric Point: 10.0801

>AT114641 WP_003873388.1 NZ_CP033910:c1904447-1904139 [Mycobacterium avium subsp. paratuberculosis]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVAQYRLRELREAAGYTQTTLAAAIGVGQNRVSQMEHGDLGTSRVDTLRKYVE
ATGGELEVSVKRPDGSRVLLSL

Download         Length: 309 bp

>AT114641 NZ_CP033910:c1904447-1904139 [Mycobacterium avium subsp. paratuberculosis]
ATGACCAATCTTGATGACATGCGCCGTCGCCGTCCCGGCAACCGGACCCGTATCGACGCGATCAAGGCGGAGATGGACCG
CGAGGTTGCCCAGTACCGTCTCCGCGAACTGCGCGAGGCTGCCGGCTACACGCAGACGACTCTCGCTGCGGCTATTGGCG
TCGGGCAGAACCGCGTCTCGCAGATGGAACATGGCGATCTGGGCACGAGCCGGGTCGACACTCTCCGCAAGTACGTGGAA
GCGACTGGGGGTGAGCTGGAAGTGTCCGTCAAGCGTCCGGACGGGTCACGGGTTCTCCTCAGCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB X7V9P5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4U5M0

References