Detailed information of TA system TA114592

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4490272..4490643
• Replicon: chromosome
• Accession: NZ_CP033884
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: EGM66_RS24220
• Protein ID: WP_001443846.1
• Coordinates: 4490494..4490643 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4490272..4490450 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS24195	4486227..4487600	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
EGM66_RS24200	4487729..4488664	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
EGM66_RS24205	4488716..4489951	-	1236	WP_000040839.1	site-specific integrase	-
EGM66_RS24210	4489953..4490168	-	216	WP_000079604.1	excisionase XisR	-
EGM66_RS24215	4490268..4490456	-	189	WP_001302840.1	DUF1187 family protein	-
-	4490272..4490450	+	179	NuclAT_0	-	Antitoxin
-	4490272..4490450	+	179	NuclAT_0	-	Antitoxin
-	4490272..4490450	+	179	NuclAT_0	-	Antitoxin
-	4490272..4490450	+	179	NuclAT_0	-	Antitoxin
EGM66_RS24220	4490494..4490643	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EGM66_RS24225	4490699..4491508	-	810	WP_000166313.1	recombination protein RecT	-
EGM66_RS24230	4491501..4494101	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
EGM66_RS24235	4494203..4494478	-	276	WP_001344816.1	hypothetical protein	-
EGM66_RS24240	4494553..4494723	-	171	WP_001352098.1	conserved protein, Rac prophage	-
EGM66_RS24245	4494723..4494944	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4488716..4539144	50428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T114592 WP_001443846.1 NZ_CP033884:c4490643-4490494 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T114592 NZ_CP033884:c4490643-4490494 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT114592 NZ_CP033884:4490272-4490450 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin