Detailed information of TA system TA114585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3213827..3214521
• Replicon: chromosome
• Accession: NZ_CP033884
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: EGM66_RS17870
• Protein ID: WP_001521903.1
• Coordinates: 3214123..3214521 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: EGM66_RS17865
• Protein ID: WP_000554758.1
• Coordinates: 3213827..3214120 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS17840	3208942..3209691	+	750	WP_016233951.1	C40 family peptidase	-
EGM66_RS17845	3209748..3210101	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
EGM66_RS17850	3210180..3211892	-	1713	Protein_2962	flagellar biosynthesis protein FlhA	-
EGM66_RS17855	3211864..3212649	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
EGM66_RS17860	3212720..3213775	+	1056	WP_001226177.1	DNA polymerase IV	-
EGM66_RS17865	3213827..3214120	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EGM66_RS17870	3214123..3214521	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EGM66_RS17875	3214531..3214983	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
EGM66_RS17880	3215228..3215434	+	207	Protein_2968	RtcB family protein	-
EGM66_RS17885	3215430..3215782	+	353	Protein_2969	peptide chain release factor H	-
EGM66_RS17890	3215839..3217296	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
EGM66_RS17895	3217557..3218015	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
EGM66_RS17900	3218107..3219351	+	1245	WP_000189541.1	esterase FrsA	-
-	3218611..3218691	+	81	NuclAT_11	-	-
-	3218611..3218691	+	81	NuclAT_11	-	-
-	3218611..3218691	+	81	NuclAT_11	-	-
-	3218611..3218691	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T114585 WP_001521903.1 NZ_CP033884:3214123-3214521 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T114585 NZ_CP033884:3214123-3214521 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT114585 WP_000554758.1 NZ_CP033884:3213827-3214120 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT114585 NZ_CP033884:3213827-3214120 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3