Detailed information of TA system TA114578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2247142..2247744
• Replicon: chromosome
• Accession: NZ_CP033884
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EGM66_RS13205
• Protein ID: WP_000897305.1
• Coordinates: 2247142..2247453 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGM66_RS13210
• Protein ID: WP_000356395.1
• Coordinates: 2247454..2247744 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS13190	2243075..2243533	+	459	WP_000526135.1	IS200/IS605 family transposase	-
EGM66_RS13195	2244022..2245836	+	1815	WP_020233236.1	hypothetical protein	-
EGM66_RS13200	2246005..2246913	-	909	WP_001306651.1	alpha/beta hydrolase	-
EGM66_RS13205	2247142..2247453	+	312	WP_000897305.1	hypothetical protein	Toxin
EGM66_RS13210	2247454..2247744	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
EGM66_RS13220	2248104..2248382	+	279	WP_001306650.1	hypothetical protein	-
EGM66_RS13230	2248801..2249019	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
EGM66_RS13235	2249238..2249480	+	243	WP_021523315.1	hypothetical protein	-
EGM66_RS13240	2249662..2250591	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EGM66_RS13245	2250588..2251223	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EGM66_RS13250	2251220..2252122	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2243075..2243533	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T114578 WP_000897305.1 NZ_CP033884:2247142-2247453 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T114578 NZ_CP033884:2247142-2247453 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT114578 WP_000356395.1 NZ_CP033884:2247454-2247744 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT114578 NZ_CP033884:2247454-2247744 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7