Detailed information of TA system TA114577

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1413600..1414399
• Replicon: chromosome
• Accession: NZ_CP033884
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: D3GWU5
• Locus tag: EGM66_RS09155
• Protein ID: WP_000347272.1
• Coordinates: 1413935..1414399 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: EGM66_RS09150
• Protein ID: WP_001307405.1
• Coordinates: 1413600..1413935 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS09135	1409385..1410155	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
EGM66_RS09140	1410171..1411505	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
EGM66_RS09145	1411880..1413451	+	1572	WP_001273738.1	galactarate dehydratase	-
EGM66_RS09150	1413600..1413935	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
EGM66_RS09155	1413935..1414399	+	465	WP_000347272.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
EGM66_RS09160	1414454..1415263	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
EGM66_RS09165	1415512..1416792	+	1281	WP_001521382.1	tagatose-bisphosphate aldolase subunit KbaZ	-
EGM66_RS09170	1416815..1417288	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
EGM66_RS09175	1417299..1418078	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
EGM66_RS09180	1418068..1418946	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
EGM66_RS09185	1418964..1419398	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1404075..1414399	10324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17832.26 Da
• Isoelectric Point: 9.6924

>T114577 WP_000347272.1 NZ_CP033884:1413935-1414399 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Nucleotide

• Length: 465 bp

>T114577 NZ_CP033884:1413935-1414399 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT114577 WP_001307405.1 NZ_CP033884:1413600-1413935 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT114577 NZ_CP033884:1413600-1413935 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829KUD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7