Detailed information of TA system TA114573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 879150..879877
• Replicon: chromosome
• Accession: NZ_CP033884
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: EGM66_RS06550
• Protein ID: WP_001521139.1
• Coordinates: 879566..879877 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGM66_RS06545
• Protein ID: WP_000126294.1
• Coordinates: 879150..879569 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS06525	874235..874762	-	528	WP_001078777.1	electron transport protein HydN	-
EGM66_RS06530	874911..875921	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
EGM66_RS06535	876181..877638	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
EGM66_RS06540	877647..879071	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
EGM66_RS06545	879150..879569	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
EGM66_RS06550	879566..879877	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
EGM66_RS06555	880044..880514	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
EGM66_RS06560	880507..880917	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
EGM66_RS06565	880914..881681	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
EGM66_RS06570	881681..882223	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
EGM66_RS06575	882233..883942	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T114573 WP_001521139.1 NZ_CP033884:c879877-879566 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T114573 NZ_CP033884:c879877-879566 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT114573 WP_000126294.1 NZ_CP033884:c879569-879150 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT114573 NZ_CP033884:c879569-879150 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9