Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 59742..60244 Replicon chromosome
Accession NZ_CP033884
Organism Escherichia coli strain 50579417

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EGM66_RS02630 Protein ID WP_000767829.1
Coordinates 59742..59996 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EGM66_RS02635 Protein ID WP_001259255.1
Coordinates 59993..60244 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGM66_RS02600 54757..54984 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
EGM66_RS02605 54998..56056 - 1059 WP_000492324.1 transport protein YeeE -
EGM66_RS02610 56235..57593 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EGM66_RS02615 57583..57645 - 63 WP_010723108.1 membrane protein YoeI -
EGM66_RS02620 57860..58789 - 930 WP_023154044.1 LysR family transcriptional regulator -
EGM66_RS02625 58835..59659 - 825 WP_000754758.1 SDR family oxidoreductase -
EGM66_RS02630 59742..59996 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EGM66_RS02635 59993..60244 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EGM66_RS02640 60527..60577 + 51 WP_001364200.1 his operon leader peptide -
EGM66_RS02645 60723..61622 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
EGM66_RS02650 61628..62932 + 1305 WP_021523144.1 histidinol dehydrogenase -
EGM66_RS02655 62929..63999 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
EGM66_RS02660 63999..65066 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T114572 WP_000767829.1 NZ_CP033884:c59996-59742 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T114572 NZ_CP033884:c59996-59742 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT114572 WP_001259255.1 NZ_CP033884:c60244-59993 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT114572 NZ_CP033884:c60244-59993 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References