Detailed information of TA system TA114561

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 12701..13127
• Replicon: plasmid p50579417_4
• Accession: NZ_CP033881
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EGM66_RS00770
• Protein ID: WP_001312861.1
• Coordinates: 12701..12859 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	12903..13127 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS00730	7787..8026	-	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-
EGM66_RS00735	8134..8781	-	648	WP_000332523.1	transcriptional regulator TraJ family protein	-
EGM66_RS00740	8973..9356	-	384	WP_001151529.1	relaxosome protein TraM	-
EGM66_RS00745	9689..10279	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
EGM66_RS00750	10576..11397	-	822	WP_001234445.1	DUF945 domain-containing protein	-
EGM66_RS00755	11508..11804	-	297	WP_001272251.1	hypothetical protein	-
EGM66_RS00765	12196..12400	+	205	Protein_19	pilus protein	-
EGM66_RS00770	12701..12859	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	12903..13127	-	225	NuclAT_0	-	Antitoxin
-	12903..13127	-	225	NuclAT_0	-	Antitoxin
-	12903..13127	-	225	NuclAT_0	-	Antitoxin
-	12903..13127	-	225	NuclAT_0	-	Antitoxin
EGM66_RS27905	12939..13118	+	180	WP_001309233.1	hypothetical protein	-
EGM66_RS00775	13139..13858	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
EGM66_RS00780	13855..14289	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EGM66_RS00785	14358..16322	-	1965	WP_060578230.1	ParB/RepB/Spo0J family partition protein	-
EGM66_RS00790	16383..16616	-	234	WP_000006003.1	DUF905 family protein	-
EGM66_RS00795	16674..17201	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
EGM66_RS00805	17503..17946	+	444	WP_024187997.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..61890	61890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T114561 WP_001312861.1 NZ_CP033881:c12859-12701 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T114561 NZ_CP033881:c12859-12701 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT114561 NZ_CP033881:c13127-12903 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin