Detailed information of TA system TA114552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3136..3721
• Replicon: plasmid p597A-14.8
• Accession: NZ_CP033871
• Organism: Acinetobacter baumannii strain MRSN15313

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: EGM95_RS20715
• Protein ID: WP_000897309.1
• Coordinates: 3401..3721 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGM95_RS20710
• Protein ID: WP_000369780.1
• Coordinates: 3136..3408 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM95_RS20690	196..1119	+	924	WP_033107831.1	replication initiation protein RepM	-
EGM95_RS20695	1204..1737	+	534	WP_033107830.1	plasmid replication DNA-binding protein	-
EGM95_RS20705	2001..2519	-	519	WP_000447193.1	sel1 repeat family protein	-
EGM95_RS20710	3136..3408	-	273	WP_000369780.1	helix-turn-helix domain-containing protein	Antitoxin
EGM95_RS20715	3401..3721	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGM95_RS20720	3908..4105	-	198	WP_000476222.1	hypothetical protein	-
EGM95_RS20725	4172..4399	-	228	WP_000921865.1	hypothetical protein	-
EGM95_RS20730	4497..4712	-	216	WP_000071892.1	cold-shock protein	-
EGM95_RS20735	5619..6560	+	942	WP_005133524.1	hypothetical protein	-
EGM95_RS20740	6612..6803	-	192	WP_032007734.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..14807	14807

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T114552 WP_000897309.1 NZ_CP033871:c3721-3401 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T114552 NZ_CP033871:c3721-3401 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10122.63 Da
• Isoelectric Point: 8.6582

>AT114552 WP_000369780.1 NZ_CP033871:c3408-3136 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT114552 NZ_CP033871:c3408-3136 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAAGGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGCGCGAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGTCCTGCAGCGACTTTAATT
CGTATTTTAGATGCTCATCCAACTTTAATTTAA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3