Detailed information of TA system TA114551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3147..3732
• Replicon: plasmid p597A-6.7
• Accession: NZ_CP033870
• Organism: Acinetobacter baumannii strain MRSN15313

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: EGM95_RS20655
• Protein ID: WP_000897309.1
• Coordinates: 3412..3732 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGM95_RS20650
• Protein ID: WP_000369780.1
• Coordinates: 3147..3419 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM95_RS20635	142..1092	+	951	WP_001205343.1	replication initiation protein RepM	-
EGM95_RS20640	1085..1660	+	576	WP_001096616.1	helix-turn-helix domain-containing protein	-
EGM95_RS21095	1680..1823	+	144	WP_001125246.1	hypothetical protein	-
EGM95_RS20645	2012..2530	-	519	WP_000447193.1	sel1 repeat family protein	-
EGM95_RS20650	3147..3419	-	273	WP_000369780.1	helix-turn-helix domain-containing protein	Antitoxin
EGM95_RS20655	3412..3732	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGM95_RS20660	3919..4116	-	198	WP_000476222.1	hypothetical protein	-
EGM95_RS20665	4183..4410	-	228	WP_000921865.1	hypothetical protein	-
EGM95_RS20670	4508..4723	-	216	WP_000071892.1	cold-shock protein	-
EGM95_RS21100	5055..5228	-	174	WP_001282484.1	hypothetical protein	-
EGM95_RS20675	5228..5599	-	372	WP_000504218.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..6667	6667

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T114551 WP_000897309.1 NZ_CP033870:c3732-3412 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T114551 NZ_CP033870:c3732-3412 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10122.63 Da
• Isoelectric Point: 8.6582

>AT114551 WP_000369780.1 NZ_CP033870:c3419-3147 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT114551 NZ_CP033870:c3419-3147 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAAGGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGCGCGAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGTCCTGCAGCGACTTTAATT
CGTATTTTAGATGCTCATCCAACTTTAATTTAA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3