Detailed information of TA system TA114525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1237217..1237807
• Replicon: chromosome
• Accession: NZ_CP033861
• Organism: Alcaligenes faecalis strain FDAARGOS_491

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0A2N9E1
• Locus tag: EGY22_RS05600
• Protein ID: WP_035268575.1
• Coordinates: 1237511..1237807 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: EGY22_RS05595
• Protein ID: WP_042484646.1
• Coordinates: 1237217..1237504 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY22_RS05580	1233004..1234251	+	1248	WP_042484654.1	D-amino acid dehydrogenase	-
EGY22_RS05585	1234450..1235460	-	1011	WP_042484652.1	acyltransferase family protein	-
EGY22_RS05590	1235739..1237106	+	1368	WP_042484649.1	DNA recombination protein RmuC	-
EGY22_RS05595	1237217..1237504	-	288	WP_042484646.1	HigA family addiction module antidote protein	Antitoxin
EGY22_RS05600	1237511..1237807	-	297	WP_035268575.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGY22_RS05605	1237941..1240454	-	2514	WP_042484643.1	EAL domain-containing protein	-
EGY22_RS05610	1240684..1240962	+	279	WP_042484640.1	acylphosphatase	-
EGY22_RS05615	1241119..1242015	+	897	WP_042484636.1	MurR/RpiR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11108.48 Da
• Isoelectric Point: 9.3459

>T114525 WP_035268575.1 NZ_CP033861:c1237807-1237511 [Alcaligenes faecalis]
MIKSFRHKGLQAYFETGSKSGIQAKHAVRLQIQLTALHSATQAEDMNAPGWKLHKLSGKNPKGQSVQDHYTISVSGNWRL
TFYFEGEDAVLVDYQDYH

Nucleotide

• Length: 297 bp

>T114525 NZ_CP033861:c1237807-1237511 [Alcaligenes faecalis]
ATGATTAAGAGCTTCCGACACAAGGGTCTTCAGGCTTACTTTGAAACCGGCTCCAAATCCGGCATTCAAGCCAAGCATGC
TGTTCGCCTTCAGATTCAGTTAACGGCCCTGCACTCTGCTACTCAAGCGGAAGATATGAACGCCCCCGGATGGAAGCTCC
ACAAGTTATCTGGCAAAAACCCCAAAGGCCAATCTGTACAAGATCACTACACAATATCGGTTAGCGGAAACTGGCGTCTA
ACCTTTTACTTTGAAGGCGAAGACGCTGTTTTGGTGGACTACCAGGACTATCACTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10430.96 Da
• Isoelectric Point: 9.8929

>AT114525 WP_042484646.1 NZ_CP033861:c1237504-1237217 [Alcaligenes faecalis]
IMRMHNPSHPGPVLREYLGELSVTEAASRLGVTRAALSRILNGSAGISADMALRLRDALGTSAEMWINMQSHYDLWQAEQ
KPRPKVVSFHGPAHA

Nucleotide

• Length: 288 bp

>AT114525 NZ_CP033861:c1237504-1237217 [Alcaligenes faecalis]
ATTATGCGTATGCACAACCCATCCCACCCCGGCCCGGTCTTGCGGGAATACTTGGGCGAACTCAGTGTCACTGAGGCGGC
ATCCCGCCTTGGCGTAACGCGTGCAGCCTTATCGCGCATTCTCAATGGTTCTGCCGGTATTTCGGCAGACATGGCCTTAC
GCCTGCGCGACGCCCTTGGAACCAGTGCTGAAATGTGGATCAACATGCAGTCCCACTACGATCTTTGGCAGGCAGAGCAA
AAGCCAAGGCCCAAAGTCGTGTCCTTTCATGGACCTGCTCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0A2N9E1