Detailed information of TA system TA114523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 26117..26775
• Replicon: plasmid unnamed1
• Accession: NZ_CP033857
• Organism: Acinetobacter sp. FDAARGOS_493

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EGY13_RS00190
• Protein ID: WP_000312250.1
• Coordinates: 26117..26476 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGY13_RS00195
• Protein ID: WP_001096429.1
• Coordinates: 26476..26775 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY13_RS00155	21638..21952	-	315	WP_000708714.1	hypothetical protein	-
EGY13_RS00165	23007..23765	-	759	WP_001053127.1	hypothetical protein	-
EGY13_RS00170	23832..24215	-	384	WP_000654348.1	hypothetical protein	-
EGY13_RS00175	24354..25052	-	699	WP_000873188.1	hypothetical protein	-
EGY13_RS00180	25119..25301	-	183	WP_000373385.1	hypothetical protein	-
EGY13_RS00185	25350..25916	-	567	WP_000710385.1	hypothetical protein	-
EGY13_RS00190	26117..26476	+	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGY13_RS00195	26476..26775	+	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
EGY13_RS00205	27313..27849	-	537	WP_000731978.1	hypothetical protein	-
EGY13_RS00210	27899..28453	-	555	WP_000790084.1	hypothetical protein	-
EGY13_RS00215	28473..28691	-	219	WP_001043201.1	hypothetical protein	-
EGY13_RS00220	28731..29360	-	630	WP_000701003.1	hypothetical protein	-
EGY13_RS00225	29377..29556	-	180	WP_000387630.1	hypothetical protein	-
EGY13_RS00230	29532..30104	-	573	WP_000443897.1	hypothetical protein	-
EGY13_RS00235	30109..30366	-	258	WP_000834290.1	hypothetical protein	-
EGY13_RS00240	30471..31751	-	1281	WP_001093570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..67023	67023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T114523 WP_000312250.1 NZ_CP033857:26117-26476 [Acinetobacter sp. FDAARGOS_493]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T114523 NZ_CP033857:26117-26476 [Acinetobacter sp. FDAARGOS_493]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT114523 WP_001096429.1 NZ_CP033857:26476-26775 [Acinetobacter sp. FDAARGOS_493]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT114523 NZ_CP033857:26476-26775 [Acinetobacter sp. FDAARGOS_493]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA