Detailed information of TA system TA114510

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 3865054..3865425
• Replicon: chromosome
• Accession: NZ_CP033850
• Organism: Escherichia coli strain FDAARGOS_497

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: EGY17_RS20630
• Protein ID: WP_001317028.1
• Coordinates: 3865231..3865425 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	3865054..3865232 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY17_RS20605	3861009..3862382	+	1374	WP_022645642.1	ATP-dependent RNA helicase DbpA	-
EGY17_RS20610	3862511..3863446	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
EGY17_RS20615	3863498..3864733	-	1236	WP_000040858.1	site-specific integrase	-
EGY17_RS20620	3864735..3864950	-	216	WP_000079604.1	excisionase XisR	-
EGY17_RS20625	3865029..3865238	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	3865054..3865232	+	179	NuclAT_0	-	Antitoxin
-	3865054..3865232	+	179	NuclAT_0	-	Antitoxin
-	3865054..3865232	+	179	NuclAT_0	-	Antitoxin
-	3865054..3865232	+	179	NuclAT_0	-	Antitoxin
EGY17_RS20630	3865231..3865425	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
EGY17_RS20635	3865482..3866291	-	810	WP_000166319.1	recombination protein RecT	-
EGY17_RS20640	3866284..3868884	-	2601	WP_108188719.1	exodeoxyribonuclease VIII	-
EGY17_RS20645	3868986..3869261	-	276	WP_001314664.1	hypothetical protein	-
EGY17_RS20650	3869336..3869512	-	177	WP_032249825.1	hypothetical protein	-
EGY17_RS20655	3869506..3869727	-	222	WP_000560230.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3856239..3908404	52165

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T114510 WP_001317028.1 NZ_CP033850:c3865425-3865231 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T114510 NZ_CP033850:c3865425-3865231 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT114510 NZ_CP033850:3865054-3865232 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin