Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 2856455..2857134 Replicon chromosome
Accession NZ_CP033850
Organism Escherichia coli strain FDAARGOS_497

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag EGY17_RS15625 Protein ID WP_000057541.1
Coordinates 2856455..2856757 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag EGY17_RS15630 Protein ID WP_000806442.1
Coordinates 2856793..2857134 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY17_RS15605 2851718..2852938 - 1221 WP_022645286.1 fosmidomycin MFS transporter -
EGY17_RS15610 2853156..2854808 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
EGY17_RS15615 2854845..2855324 - 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
EGY17_RS15620 2855528..2856322 - 795 WP_022645287.1 TraB/GumN family protein -
EGY17_RS15625 2856455..2856757 + 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EGY17_RS15630 2856793..2857134 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
EGY17_RS15635 2857192..2859696 - 2505 WP_022645288.1 copper-exporting P-type ATPase CopA -
EGY17_RS15640 2859959..2860891 + 933 WP_022645289.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2856455..2865735 9280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T114505 WP_000057541.1 NZ_CP033850:2856455-2856757 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T114505 NZ_CP033850:2856455-2856757 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCTGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT114505 WP_000806442.1 NZ_CP033850:2856793-2857134 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT114505 NZ_CP033850:2856793-2857134 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References