Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2632688..2633382 Replicon chromosome
Accession NZ_CP033850
Organism Escherichia coli strain FDAARGOS_497

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag EGY17_RS14575 Protein ID WP_001263491.1
Coordinates 2632984..2633382 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag EGY17_RS14570 Protein ID WP_000554755.1
Coordinates 2632688..2632981 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY17_RS14545 2627724..2627984 - 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -
EGY17_RS14550 2628170..2628943 + 774 WP_022645219.1 C40 family peptidase -
EGY17_RS14555 2629041..2630753 - 1713 Protein_2440 flagellar biosynthesis protein FlhA -
EGY17_RS14560 2630725..2631510 + 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
EGY17_RS14565 2631581..2632636 + 1056 WP_022645222.1 DNA polymerase IV -
EGY17_RS14570 2632688..2632981 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EGY17_RS14575 2632984..2633382 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EGY17_RS14580 2633392..2633844 + 453 WP_023909048.1 GNAT family N-acetyltransferase -
EGY17_RS14585 2634035..2635174 + 1140 WP_022645224.1 RNA ligase RtcB family protein -
EGY17_RS14590 2635171..2635785 + 615 WP_022645225.1 peptide chain release factor H -
EGY17_RS14595 2635847..2636356 + 510 WP_001361775.1 hydrolase -
EGY17_RS14600 2636373..2636654 - 282 WP_022645226.1 hypothetical protein -
EGY17_RS14605 2636663..2638120 - 1458 WP_022645227.1 cytosol nonspecific dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T114503 WP_001263491.1 NZ_CP033850:2632984-2633382 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T114503 NZ_CP033850:2632984-2633382 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT114503 WP_000554755.1 NZ_CP033850:2632688-2632981 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT114503 NZ_CP033850:2632688-2632981 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References