Detailed information of TA system TA114498

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 2273732..2274144
• Replicon: chromosome
• Accession: NZ_CP033850
• Organism: Escherichia coli strain FDAARGOS_497

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: EGY17_RS12900
• Protein ID: WP_000132601.1
• Coordinates: 2273732..2274073 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	2274068..2274144 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY17_RS12890	2271145..2272191	-	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-
EGY17_RS12895	2272191..2273570	-	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
EGY17_RS12900	2273732..2274073	-	342	WP_000132601.1	endoribonuclease SymE	Toxin
-	2274068..2274144	+	77	NuclAT_8	-	Antitoxin
-	2274068..2274144	+	77	NuclAT_8	-	Antitoxin
-	2274068..2274144	+	77	NuclAT_8	-	Antitoxin
-	2274068..2274144	+	77	NuclAT_8	-	Antitoxin
-	2274068..2274144	+	77	NuclAT_9	-	Antitoxin
-	2274068..2274144	+	77	NuclAT_9	-	Antitoxin
-	2274068..2274144	+	77	NuclAT_9	-	Antitoxin
-	2274068..2274144	+	77	NuclAT_9	-	Antitoxin
EGY17_RS12905	2274301..2275695	-	1395	WP_001272445.1	type I restriction-modification system specificity subunit	-
EGY17_RS12910	2275692..2277281	-	1590	WP_001063200.1	type I restriction-modification system methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2261441..2275695	14254

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T114498 WP_000132601.1 NZ_CP033850:c2274073-2273732 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T114498 NZ_CP033850:c2274073-2273732 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT114498 NZ_CP033850:2274068-2274144 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin