Detailed information of TA system TA114493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1646737..1647339
• Replicon: chromosome
• Accession: NZ_CP033850
• Organism: Escherichia coli strain FDAARGOS_497

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EGY17_RS09770
• Protein ID: WP_000897305.1
• Coordinates: 1646737..1647048 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGY17_RS09775
• Protein ID: WP_000356397.1
• Coordinates: 1647049..1647339 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY17_RS09740	1641767..1642552	+	786	WP_000059679.1	DeoR/GlpR family DNA-binding transcription regulator	-
EGY17_RS09745	1642651..1643250	+	600	WP_001314324.1	glucose-1-phosphatase	-
EGY17_RS09750	1643244..1644116	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EGY17_RS09755	1644113..1644550	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EGY17_RS09760	1644595..1645536	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EGY17_RS09765	1645600..1646508	-	909	WP_001318161.1	alpha/beta hydrolase	-
EGY17_RS09770	1646737..1647048	+	312	WP_000897305.1	hypothetical protein	Toxin
EGY17_RS09775	1647049..1647339	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EGY17_RS09785	1647944..1648162	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
EGY17_RS09790	1648377..1649306	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
EGY17_RS09795	1649303..1649938	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EGY17_RS09800	1649935..1650837	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T114493 WP_000897305.1 NZ_CP033850:1646737-1647048 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T114493 NZ_CP033850:1646737-1647048 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT114493 WP_000356397.1 NZ_CP033850:1647049-1647339 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT114493 NZ_CP033850:1647049-1647339 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7