Detailed information of TA system TA114442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2024462..2025057
• Replicon: chromosome
• Accession: NZ_CP033833
• Organism: Pseudomonas aeruginosa strain FDAARGOS_571

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: EGY26_RS09885
• Protein ID: WP_003113526.1
• Coordinates: 2024779..2025057 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGY26_RS09880
• Protein ID: WP_003133769.1
• Coordinates: 2024462..2024767 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY26_RS09865	2021184..2022047	-	864	WP_023098850.1	integrase domain-containing protein	-
EGY26_RS09870	2022648..2023790	-	1143	WP_023098851.1	hypothetical protein	-
EGY26_RS09880	2024462..2024767	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
EGY26_RS09885	2024779..2025057	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGY26_RS09895	2025386..2027614	+	2229	WP_023104079.1	TonB-dependent receptor	-
EGY26_RS09900	2027685..2028332	-	648	WP_003095021.1	carbonate dehydratase	-
EGY26_RS09905	2028394..2029632	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T114442 WP_003113526.1 NZ_CP033833:c2025057-2024779 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T114442 NZ_CP033833:c2025057-2024779 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT114442 WP_003133769.1 NZ_CP033833:c2024767-2024462 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT114442 NZ_CP033833:c2024767-2024462 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3