Detailed information of TA system TA114433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 315502..316097
• Replicon: chromosome
• Accession: NZ_CP033832
• Organism: Pseudomonas aeruginosa strain FDAARGOS_505

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: EGY23_RS01555
• Protein ID: WP_003117425.1
• Coordinates: 315819..316097 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGY23_RS01550
• Protein ID: WP_003099268.1
• Coordinates: 315502..315807 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY23_RS01515	310955..311245	-	291	WP_023083237.1	DUF5447 family protein	-
EGY23_RS01525	311457..311729	-	273	WP_003115921.1	hypothetical protein	-
EGY23_RS01530	311839..312105	+	267	WP_016852153.1	hypothetical protein	-
EGY23_RS01535	312279..313073	+	795	WP_023082695.1	helix-turn-helix domain-containing protein	-
EGY23_RS01540	313048..314586	+	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
EGY23_RS01545	314598..315125	-	528	WP_071535723.1	AAA family ATPase	-
EGY23_RS01550	315502..315807	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
EGY23_RS01555	315819..316097	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGY23_RS01565	316426..318654	+	2229	WP_003099265.1	TonB-dependent receptor	-
EGY23_RS01570	318724..319371	-	648	WP_003095021.1	carbonate dehydratase	-
EGY23_RS01575	319433..320671	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T114433 WP_003117425.1 NZ_CP033832:c316097-315819 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T114433 NZ_CP033832:c316097-315819 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT114433 WP_003099268.1 NZ_CP033832:c315807-315502 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT114433 NZ_CP033832:c315807-315502 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5