Detailed information of TA system TA114294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 48497..49148
• Replicon: plasmid unnamed1
• Accession: NZ_CP033769
• Organism: Acinetobacter baumannii strain FDAARGOS_533

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: EGX83_RS18400
• Protein ID: WP_000269904.1
• Coordinates: 48792..49148 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGX83_RS18395
• Protein ID: WP_024436604.1
• Coordinates: 48497..48799 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX83_RS18360	43742..44446	+	705	WP_004741406.1	IS6-like element IS1006 family transposase	-
EGX83_RS18365	44486..44728	+	243	WP_024436969.1	recombinase family protein	-
EGX83_RS18370	44953..45381	-	429	WP_016653412.1	hypothetical protein	-
EGX83_RS18375	45578..46912	+	1335	WP_025469452.1	ISNCY family transposase	-
EGX83_RS18380	47051..47563	-	513	WP_024436968.1	SMI1/KNR4 family protein	-
EGX83_RS18385	47602..48120	-	519	WP_000447196.1	sel1 repeat family protein	-
EGX83_RS18390	48295..48504	-	210	WP_000069475.1	hypothetical protein	-
EGX83_RS18395	48497..48799	-	303	WP_024436604.1	XRE family transcriptional regulator	Antitoxin
EGX83_RS18400	48792..49148	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGX83_RS18405	49865..51919	+	2055	WP_004740259.1	TonB-dependent receptor	-
EGX83_RS18410	52033..52719	+	687	WP_004740261.1	DUF4145 domain-containing protein	-
EGX83_RS18415	52761..52868	+	108	Protein_53	transcriptional regulator	-
EGX83_RS18420	53066..53401	+	336	WP_004740263.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	katA	1..97783	97783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T114294 WP_000269904.1 NZ_CP033769:c49148-48792 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T114294 NZ_CP033769:c49148-48792 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAATGAATGGCTTGAACAGCAAGATGAAGCAACACAAGAGAAAGTCTTAGC
TGCATTGGTTGTTCTACAACAGCAAGGGCCTAGTTTAGGTCGTCCTTTAGTAGATACCGTCTACGATTCTAAATTTACCA
ATATGAAGGAACTACGTGTTCAGCATCGTGGTAAACCGTTAAGGGCATTTTTTGCATTCGATCCCCTAAGACAAGCGATT
GTGCTGTGTATTGGTGATAAGAGTAATAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGCACT
TCATCTCTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11181.00 Da
• Isoelectric Point: 7.3198

>AT114294 WP_024436604.1 NZ_CP033769:c48799-48497 [Acinetobacter baumannii]
MAKSLQELLASRSPESQTRIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT114294 NZ_CP033769:c48799-48497 [Acinetobacter baumannii]
ATGGCTAAGAGCTTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAACTCGTATTCAAAAAATGGCTGATGAATT
ACTTTTAGAAAGTCAGCTTCACCTTATCCGAGAAGAGTTAGAAATTTCCCAGAAAGAACTAGCTGCAACCCTTGGAATTA
AACAGCCGTCATTATCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGTGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGGTTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1