Detailed information of TA system TA114290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4750125..4750819
• Replicon: chromosome
• Accession: NZ_CP033762
• Organism: Escherichia coli strain FDAARGOS_536

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: EGX93_RS24285
• Protein ID: WP_001263500.1
• Coordinates: 4750421..4750819 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: EGX93_RS24280
• Protein ID: WP_000554758.1
• Coordinates: 4750125..4750418 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX93_RS24255	4745942..4746409	+	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-
EGX93_RS24260	4746429..4747145	+	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
EGX93_RS24265	4747158..4748021	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
EGX93_RS24270	4748024..4748947	+	924	WP_060578024.1	putative lateral flagellar export/assembly protein LafU	-
EGX93_RS24275	4749018..4750073	+	1056	WP_001226168.1	DNA polymerase IV	-
EGX93_RS24280	4750125..4750418	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EGX93_RS24285	4750421..4750819	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EGX93_RS24290	4750829..4751281	+	453	WP_001059897.1	GNAT family N-acetyltransferase	-
EGX93_RS24295	4751471..4752610	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
EGX93_RS24300	4752607..4753221	+	615	WP_000602135.1	peptide chain release factor H	-
EGX93_RS24305	4753278..4754735	-	1458	WP_042018326.1	cytosol nonspecific dipeptidase	-
EGX93_RS24310	4754996..4755454	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T114290 WP_001263500.1 NZ_CP033762:4750421-4750819 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T114290 NZ_CP033762:4750421-4750819 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT114290 WP_000554758.1 NZ_CP033762:4750125-4750418 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT114290 NZ_CP033762:4750125-4750418 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3