Detailed information of TA system TA114284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3763955..3764557
• Replicon: chromosome
• Accession: NZ_CP033762
• Organism: Escherichia coli strain FDAARGOS_536

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: EGX93_RS19380
• Protein ID: WP_000897302.1
• Coordinates: 3763955..3764266 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EGX93_RS19385
• Protein ID: WP_000356397.1
• Coordinates: 3764267..3764557 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGX93_RS19355	3759869..3760468	+	600	WP_001315111.1	glucose-1-phosphatase	-
EGX93_RS19360	3760462..3761334	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EGX93_RS19365	3761331..3761768	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EGX93_RS19370	3761813..3762754	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EGX93_RS19375	3762818..3763726	-	909	WP_001331553.1	alpha/beta hydrolase	-
EGX93_RS19380	3763955..3764266	+	312	WP_000897302.1	hypothetical protein	Toxin
EGX93_RS19385	3764267..3764557	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EGX93_RS19390	3764642..3764854	+	213	WP_000197774.1	hypothetical protein	-
EGX93_RS19395	3764916..3765194	+	279	WP_001296612.1	hypothetical protein	-
EGX93_RS19405	3765590..3765808	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
EGX93_RS19410	3766031..3766960	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
EGX93_RS19415	3766957..3767592	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EGX93_RS19420	3767589..3768491	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T114284 WP_000897302.1 NZ_CP033762:3763955-3764266 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T114284 NZ_CP033762:3763955-3764266 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT114284 WP_000356397.1 NZ_CP033762:3764267-3764557 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT114284 NZ_CP033762:3764267-3764557 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416